Quantification of Gallic Acid and Ellagic Acid from the Seed of Covnus officinalis by UHPLC Method and Their Antioxidant Activity

摘要

Gallic acid and ellagic acid have been identified in the seed ofCornus officinalis by the use of an ultrahigh performance liquid chromatography (UHPLC) method coupled with a diode array detector (DAD). The water extract of C. officinalis seed contained the highest gallic acid content (3.03 ±0.10mg/g seed), which was followed by aqueous methanol extract (2.43 ± 0.10 mg/g seed) and aqueous etlianol extract (1.53±0.25mg/g seed). But a higher concentration (12.32±0.45mg/g seed) of ellagic acid was obtained from extraction with aqueous methanol than with aqueous ethanol (11.03±0.42mg/g seed) and water (7.28±0.16mg/g seed). After heat treatment and acid hydrolysis, C. officinalis seed had higher concentrations of gallic acid and ellagic acid, contributing to more potent antioxidant activity. The results demonstrated a rich source of gallic acid and ellagic acid in C. officinalis seed, which might provide a novel source of these natural antioxidants.