Sex Identification of Red-crowned Crane by the Polymerase Chain Reaction

摘要

Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane' s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.