A Comparison of Techniques for Preparing Somatic Chromosomes in Ribes Species

摘要

Ribes plants, like most of other Fruit trees, arc characterized by their large number but small size of somatic chromosomes. In the light of these special characteristics, we have examined several factorial combinations of material, prctrcatmcnt, slide preparing method and stain to identify the most promising tcchique for studying the chromosomes and further analyzing the karyotypcs and chromosome banding of Ribes plants. The resuts indicated that the combination of root tip with prctrcatmcnt of 2 mmolL~(-1) 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3~5 hours at 14 deg C prc-hypotonic treatment of 0.07mol L~(-1) KCl for 30 min, fixation in Carnoy's fluid, hydrolysis in 5% ccllulasc and 5% pcctinasc mixture for 4~ 5 hours at 25deg C, post-hypotonic treatment in distilled water for half an hour and staining in Gicmsa could make the chromosome preparation superior to other treatment combinations.