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Identification of molecular markers linked to TuMV resistant gene in cabbage.

机译:鉴定与甘蓝中 TuMV 抗性基因相关的分子标记。

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A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA (Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.
机译:从1047(易感)和A21(抗性)之间的杂交中获得了总共144个F 2 个体。使用BSA(Bulked Segregant Analysis)在200个随机引物中筛选出两个RAPD标记。与 TuMV 抗性基因分开的两个RAPD标记分别为AG13 / 2000和U16 / 660,分别为7.7 cM和8.38 cM。将两个RAPD片段转换为SCAR标记。 SCAR标记仅限于种质。

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