The determination of canthaxanthin in feed ingredients by high performance liquid chromatography.

摘要

A series of studies were conducted to establish a methodology for the accurate and efficient determination of canthaxanthin in feed ingredients. Agilent ZORBAX Eclipse DB-C18 analytical column (4.6x150 mm, 5 micro m) was used and kept at 25 degrees C. The mobile phase was acetonitrile:methanol (v/v)=95:5 and eluted compounds were detected by DAD absorbance (470 nm). The flow rate was maintained at 1.0 ml/min. The response of the method was linear over the range 1.0-20.0 micro g/ml canthaxanthin assay solution (R2=0.9998). Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103%. Variation coefficient was less than 3.53%. The method was found simple, precise, sensitive and reproducible.