Study on cell suspension culture of floribunda rose.

摘要

Friable callus was induced when immature seeds of floribunda rose (Rosa floribunda [R. helenae]) were inoculated on MS medium supplemented with 2,4-D at 3.0 mg litre-1. When transferred onto subculture media, friable callus developed into embryogenic callus, which was used to establish cell suspension lines. Cell suspensions had to be subcultured at an interval of 4-5 days at the first several culture cycles. The best subculturing cycle for the stable cell suspensions was 8-10 days. The best inoculum quantity was 1 ml PCV (Packed Cell Volume) per 40 ml culture fluid.