RAPD analysis on the four kinds of color-flowered Salvia splendens Ker-Gawl

摘要

In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture (25 μL total volume) that contained 2.0 μL 10 x buffer, 0.45 mmol·L~(-1) dNTPs, 2.0 mmol· L~(-1) Mg~(2+), 2 U Taq DNA polymerase, 0.30 umol· L~(-1) primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.