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A NEW CONFOCAL SCANNING BEAM LASER MACROSCOPE USING A TELECENTRIC, F-THETA LASER SCAN LENS

机译:使用远心F-THETA激光扫描透镜的新型共聚焦扫描激光束显微镜

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摘要

A new confocal scanning beam system (MACROscope) that images very large-area specimens is described. The MACROscope uses a telecentric, f-theta laser scan lens as an objective lens to image specimens as large as 7.5cm x 7.5cm in 5s. The lateral resolution of the MACROscope is 5 mu m and the axial resolution is 200 mu m. When combined with a confocal microscope, a new hybrid imaging system is produced that uses the advantages of small-area, high-speed, high-resolution microscopy (0.2 mu m lateral and 0.4 mu m axial resolution) with the large-area, high-speed, good-resolution imaging of the MACROscope, The advantages of the microscope/MACROscope are illustrated in applications which include reflected-light confocal images of biological specimens, DNA sequencing gels, latent fingerprints and photoluminescence imaging of porous silicon. [References: 10]
机译:描述了一种新的共聚焦扫描光束系统(MACROscope),可以对非常大面积的样本成像。 MACROscope使用远心f-theta激光扫描透镜作为物镜,可以在5秒钟内对7.5cm x 7.5cm的标本成像。 MACROscope的横向分辨率为5μm,轴向分辨率为200μm。当与共焦显微镜结合使用时,将产生一种新的混合成像系统,该系统利用了小面积,高速,高分辨率显微镜(横向分辨率为0.2μm,轴向分辨率为0.4μm)和大面积,高分辨率的优势。 MACROscope的高速,高分辨率成像。显微镜/ MACROscope的优势在以下应用中得到了展示,包括生物标本的反射光共聚焦图像,DNA测序凝胶,潜在指纹和多孔硅的光致发光成像。 [参考:10]

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