Demonstration of beta-1,3-glueanase Activities in Lutoids of Hevea brasiliensis Latex

摘要

Beta-l,3-glucanase activity, generally known to be involved in plant defence reactions against pathogens, was observed in extracts of lutoids from Hevea latex. The beta-l,3-glucanase activities detected after native isoelectrofocusing (IEF) led to thevisualisation of five bands consisting of three basic isozymes (pI 10.00 ± 0.02; 9.32 ± 0.24 and 9.0 ± 0.22), and two acid isozymes (pI 5.49 ± 0.02 and4.88 ± 0.03). Immunodetection with tobaccopolyclonal antibodies against acid and basic beta-l,3-glucanases, revealed the same protein profile, characterised by three major bands with molecular weights of 38.9 ± 1.2, 36.4 ± 0.89 and 30.1 ± 1 kD, respectively. The presence of apparently constitutive chitinases and beta-1, 3-glucanases in lutoids is discussed.