Biosynthesis of Panaefluoroline B from the Cultured Mycobiont of Amygdalaria panaeola

摘要

Panaefluoroline B (2) is a fluorescent yellowish-green pigment produced by the cultured mycobiont of a lichen, Amygdalaria panaeola. Panaefluoroline B (2) has an isoquinoline skeleton, a C-5 unit, and an amino acid, glycine, in its structure. The biosynthetic pathway of 2 was revealed by feeding experiments using [1-C-13]-sodium acetate and [1,2-C-13(2)] [N-15]-glycine. The analysis of labeling patterns of 2 and its mass spectrum suggested the isoquinoline part is biosynthesized via the acetate-malonate pathway with glycine as the nitrogen source and that the C-5 unit originates from the mevalonate pathway.