Efficacy and Defence Response of a Bioformulation against Leaf Folder Insect and Sheath Blight Disease of Rice

摘要

Efficacy of a bioformulation consisting of Pseudomonas fluorescens strains, Pf-1 and AH-1 (Agathir hills) and an entomopathogenic fungus Beauveria bassiana was tested against leaf folder insect (Cnaphalocrocis medinalis Guenee) and rice sheath blightdisease (Rhizoctonia solani Kuhn^ under glasshouse and field conditions. The treatment reduced the incidence of leaf folder and sheath blight disease in rice likely due to induced resistance. To understand the mechanism of induction of pest and disease resistance a protein profiling strategy was performed in leaf folder infested rice plants inoculated with/without the bioformulation. Inoculation with bioformulation had significant effect on the expression of proteins viz., ribulose-bisphosphate carboxylase, large chain precursor of rice chloroplast, putative ATP dependent CIP protease proteolytic sub unit, peroxidase component PR-2 or 4, and putative glutathione S-transferase. The results indicated potential involvement of these key genes due to bioagents-mediated plant resistance against the target insect pest and the pathogen.