Molecular Analysis of Sorghum Genotypes Differing to Drechslera Leaf Blight Disease

摘要

Genetic diversity among 15 sorghum {Sorghum bicolor L.) genotypes was evaluated using RAPD markers and classified them in response to Drechslera leaf blight. Total 17 primers were screened, out of which 14 primers generated Polymorphic banding pattem.The average level of polymorphism was 93 %. Based on the RAPD markers, dendrogram was constructed using the UPGMA method. The similarity coefficient ranged from 0.31 to 0.76 with an average of 0.53. The dendrogram clearly divided the 15 sorghum genotypes into six main clusters. Cluster analysis concluded that highly susceptible genotypes (EJ-4, EJ-lland E-14), susceptible genotype (EJ-12) except E-7, were found genetically similar. These genotypes were also distinct from highly resistant genotypes (PJ-1430) and resistant genotype (E-2), as depicted in the cluster analysis. A critical perusal indicated that out of 17 RAPD primers, six were found to be highly informative as they gave unique bands to differentiate various genotypes. Seven unique bands were selected for identification of disease resistant and susceptible genotypes of sorghum. It is quite optimistic to say that the study will find application in assessment of genotype identification for Drechslera leaf blight.