Molecular Cloning and Characterization of Yellow Mosaic Virus from Mung Bean from Northern Region of Tamil Nadu Indicates Association of Mungbean Yellow Mosaic India Virus DNA A with a Recombinant DNA B

摘要

Genomic components of the yellow mosaic virus, the causal agent of yellow mosaic disease in mung bean were cloned and sequenced from an infected sample of mung bean. Nucleotide sequence analysis of DNA A component revealed more than 98 per cent identity with mung bean yellow mosaic India virus (MYMTV) isolate from West Bengal (Gene bank acc no:HF92262) and therefore designated as an isolate of MYMIV-[iN::Vrng:Mg:13-A] whereas analysis of DNA B component revealed more than 93 per cent identity with some DNA B variant reported for mung bean yellow mosaic virus (MYMV) and mung bean yellow mosaic India virus (MYMIV) but showed very less identity (only 45%) with one set of DNA B components of MYMV.Total nucleotide and predicted amino acid sequence analysis of MYMIV-[Mg ] with other yellow mosaic isolates clearly established dichotomy of the isolates.