Molecular characterization of Xanthomonas axonopodis pv. punicae causing bacterial blight of pomegranate.

摘要

Bacterial blight caused by Xanthomonas axonopodis pv. punicae is a major disease in pomegranate (Punica granatum) cultivation in India threatening the export potential of this fruit. The present investigation describes characterization of 17 isolates of X. axonopodis pv. punicae (Xap) at molecular level using random decamer primers. The primers OPA-03, OPA-09, OPI-13, OPG-07 and OPX-11 were found to be most significant and polymorphic. Percent polymorphism was 96.87 and the average number of polymorphic bands per primer was 11.87. The dendrogram analysis based on RAPD fingerprint data revealed five major groups among the 17 isolates of this pathogen. The isolate XapNRCP1 was found more distant to all isolates, while XapNS and XapSAAJ were found 91% similar to each other. Genetic distance among all strains ranged between 9 and 42%.