Effect of Substrates on Vegetative Growth and Fruiting Induction of Ganoderma Species

摘要

The radial growth of Ganoderma spp. (Reishi mushroom) was significantly higher in malt extract agar added with linseed extract medium among from 10 media assessed. It was higher in all the strains except Ganoderma lucidum (Local strain) and G. lucidum (IS). Among all the strains, faster growth was observed in local strain of Ganoderma. The average biomass comprising fresh and dry was high in malt extract broth with the addition of sunflower extract, linseed extract or soybean extract. In local strain of Ganoderma, the reproductive structures were also noticed which were not observed in G. applanatum and G. lucidum (IS). Significantly higher radial growth of G. applanatum and G. lucidum (IS) was recorded at 30-35C. Among 13 substrates used for spawn development, the fastest growth was observed in bajra grains by all the strains. Again among the three strains, cent percent mycelial colonization was observed in local strain of Ganoderma. In this strain, fruiting induction was observed after 15 days of inoculation in bajra grains but 30 days after inoculation in sugarcane baggase and sunflower straw substrate. Fruiting induction was also observed by G. applanatum after 30 days of inoculation in sugarcane baggase and sugarcane baggase with saw dust. Among six fruiting substrates, fast mycelial growth was observed in the saw dust by G. applanatum and G. lucidum. In both the strains, pin head initiation was faster while weight of sporophore and biological efficiency were higher in wheat straw with added saw dust substrate. No pinning was observed in saw dust substrate. When sugarcane baggase substrate was also added, the mycelial growth was faster than in saw dust. In G. applanatum and local strain of Ganoderma (GR/L/04/1), pin head initiation was faster in wheat straw added with saw dust but it was delayed in sugarcane baggase. Weight of sporophore and biological efficiency was higher in wheat straw added with saw dust substrate in G. applanatum. But, in local strain of Ganoderma it was higher in paddy straw added with saw dust substrate. There was no doubt that sugarcane baggase gave good result than saw dust for mycelial growth but pinning was very late in this substrate in local strain of Ganoderma and in G. applanatum, no pinning was observed. Saw dust substrate did not show any pin head initiation. Our results indicate that wheat straw added with saw dust