Morphological, pathological and biochemical variations among Nomuraea rileyi isolates.

摘要

Six isolates of an entomopathogenic fungus Nomuraea rileyi were characterized for their morphological, cultural, pathological characteristics and their protein profile. The isolates varied considerably for morphological and cultural characteristics, such as colony colour, growth pattern, conidial production and size of conidia on SMAY (Saboraud's Maltose Agar+Yeast extract) medium. In pathogenicity test on third instar larvae of Helicoverpa armigera the local isolate Nr-PDKV isolate was found to be the most virulent causing 86.7% mortality in 120.17 h followed by Nr-DHW that caused 76.7% mortality in 132.15 h. Mycelial protein profiles of these isolates exhibited significant variations in their electrophoresis patterns. The isolates from Dharwad (Nr-DHW), Banglore (Nr-BNG) and Hyderabad (Nr-HYD) shared different similarities among themselves while Nr-PDKV and Nr-AL exhibited close similarity.