Expression of mRNA for 55-kDa and 75-kDa tumor necrosis factor (TNF) receptors in mouse cerebrovascular endothelium: effects of interleukin-1 beta, interferon-gamma and TNF-alpha on cultured cells.

摘要

The interactions between tumor necrosis factor-alpha (TNF-alpha) and its receptors have been implicated to play an important role in the pathogenesis of experimental autoimmune encephalomyelitis (EAE). The effects of cytokines on the steady state levels of TNF receptor (TNFr) mRNA in cerebrovascular endothelial cells (CVE) cultured from EAE-susceptible (SJL/J) and EAE-resistant (BALB/c) mice were examined. Interferon-gamma and interleukin-1 beta up-regulated the levels of both the 55-kDa and 75-kDa TNFr mRNA, while TNF-alpha had no effect. No differences were observed in cytokine-induced TNFr mRNA levels between BALB/c and SJL/J CVE that might explain differences in EAE susceptibility.