A novel amperometric biosensor for hydrogen peroxide and glucose based on cuprous sulfide nanoplates

摘要

A facile, greener and template free route has been developed to produce cuprous sulfide (Cu2S) nanoplates (NPs) with average diameters of 70-150 nm, via one step solvothermal decomposition of a single-source precursor (SSP) Cu(ACDC)2 [ACDC = 2-aminocyclopentene-1-dithiocarboxylatel in the presence of ethylenediamine (EN) and triethylenetetramine (TETA) as structure orienting agents. The precursor complex and nanomaterials were thoroughly characterized by several common techniques and measurements, which give the composition and characteristics of the materials. Amperometric biosensors for hydrogen peroxide (H2O2) and glucose have been constructed by immobilizing the synthesized Cu2S NPs in glutaraldehyde on a glassy carbon (GC) electrode using a direct dropcoating method. The proposed sensor has displayed faster response, high and reproducible sensitivity (64.27 μA mM~(-1)) with linear range of 10 μM to 3.75 mM, towards the electrochemical biosensing of H2O2 at -0.35 V (vs. Ag/AgCI). The sensor also showed high and reproducible sensitivity (61.67 μA mM~(-1)) towards glucose determination with linear range of 10 μM to 3.1 mM. The antiinference ability of electroactive molecules and favorable stability are some of the advantages of the proposed sensor. Finally, using the sensor we have determined the glucose concentration in a human blood serum sample. The results strongly demonstrate the usefulness of Cu2S NPs for biosensor design and other biological applications.