Nucleotide ester-forming alcoholytic activities of nucleotide phrophosphatases: impliations for practical biotransformation,enzyme mechanisms and biological function

摘要

Nucleotide pyrophosphatases (NPP) hydrolyze phosphoanhydride and phosphodiester derivatives of nucleoside 5'-monophosphates (NMR) yielding NMP as a product. In a water-alcohol mixture, the alcohol (R-OH) competes andsubstitutes for water as the splitting agent, so a mixture of NMP and NMP-O-alkyl ester (NMP-O-R) is formed. NPPs from snake venom, potato tuber and mammalian tissues have been studied in this regard. Snake and potato NPPs were considered as possible pracical biocatalysts to synthesize NMP-O-Rs from various nucleotidic substrates and alcohols. Mammalian NPPs, mainly from human blood and rat liver, were studied considering the possibility that the alcoholytic reactions catalyzed by them could be biologically relevant. Valuable information on the active centers and catalytic mechanisms of NPPs was also obtained.