Size Tunable Green Synthesis of Silver Nanoparticles Using Trachyspermum Ammi (Ajwain) and Their Effect on a B Cell Line

摘要

With the development of green nanotechnology, researchers are interested in generating new ecofriendly methods for biosynthesis of nanoparticles. In this study, we report a simple and cost effective way for size controlled biosynthesis of stable silver nanoparticles using Trachyspermum ammi (Ajwain) aqueous extract without any special reducing and capping agents. The formation of silver nanoparticles was monitored through surface plasmon behaviour as a function of time. These biosynthesized nanoparticles were characterized with the help of Transmission Electron Microscopy and Fourier Transform Infrared spectroscopy. The silver nanoparticles generated did not aggregate, suggesting that the chemicals present in the seed extract serve as excellent coatings on the nanoparticles and thus prevent them from aggregation. Different sized Ag nanoparticles have been prepared by using two different concentrations of AgNO_3 which affected their optical and biological properties. Dynamic Light Scattering, UV-Vis absorption and Photoluminescence techniques are used to study this effect. Raji cells (a B cell line) were cultured with various concentrations of above synthesised green silver nanoparticles and checked for cell viability with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.