Lipid-conjugated oligonucleotides via 'click chemistry' efficiently inhibit hepatitis C virus translation

摘要

Conjugation of a lipid moiety via "click chemistry" potentiates the cellular uptake of oligonucleotides and allows their intracellular delivery. These nontoxic lipid conjugates efficiently inhibit hepatitis C virus internal ribosome entry site (IRES)-mediated translation in human hepatic Huh7 cells. The biological activity of the lipidconjugated oligonucleotides is not affected by the presence of serum.