Properties and syntheses of copolymeric support for enzymes immobilization from vinylene carbonate and poly(vinylene alcohol) n-methylolacrylamide ether

摘要

First, Poly (vinylene alcohol) (PVA) N-methylolacrylamide ether (E-PVA) was prepared, then dissolved it with vinylene carbonate (VCA) and water-soluble initiator in water. The solution was copolymerized in paraffin oil to yield hydrophilic beads containing cyclic carbonate groups. By attachment of trypsin, properties of the supports were determined and the result shows that amount of enzymes coupled to supports and specific activity of immobilized trypsin were related to content of VCA structure unit, reaction time, concentration of enzyme solution, etc.