sonicating multi-lumen sliced catheter tips after the roll-plate technique improves the detection of catheter colonization in adults (vol 122, pg 20, 2016)

摘要

The Maid technique is the standard method for detecting catheter tip (CT) colonization. However, some "multi-lumen" catheters finish in a vaulted fornix and end at different distances from the CT. Therefore, we compared the traditional Maki technique with the sonication method using several cross-cut fragments of the CT. Our objective was to assess the yield of the Maki technique followed by sonication in the detection of adult CT colonization and catheter-related bloodstream infection (C-RBSI). For 3 months, we prospectively performed CT cultures of polyurethane catheters from adult patients admitted to our institution. First, we performed CT culture using the Maid technique on blood agar plates and then sonicated small fragments of CTs in 5 ml of BHI followed by culture of 100 I of the sonicate. We included a total of 252 CVCs, with overall colonization and C-RBSI rates of 14.3% (36/252) and 5.9% (15/252). Of the 36 colonized CVC5, 21 (58.3%) were detected both by Maid and sonication, 6 (16.7%) were detected only by Maki technique, and 9 (25.0%) only by sonication method. Among 15 episodes with concomitant bacteremia, both techniques were positive and concordant in 11 cases (73.3%) and in 4 cases (26.7%) sonication was the only positive technique.