Enzymatic monitoring of lignin and lignin derivatives biooxidation

摘要

Lignin oxidation was enzymatically monitored by measuring methanol released during the reaction. The methanol was oxidized to formaldehyde and hydrogen peroxide, and the latter used to oxidize ABTS to a product measured spectrophotometrically. The efficiency was comparable to the commonly used gas chromatography method. The assay was fast and inexpensive. (C) 2015 Elsevier B.V. All rights reserved.