An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants

摘要

A simple method to isolate, screen and select phage-resistant mutants of Bacillus thuringiensis was developed. The traditional double-layer agar method was improved by a combination of the spotting assay using a lytic phage, to generate the bacterial-resistant mutants, with an inverted spotting assay (ISA), to rapidly screen the candidate-resistant mutants. (C) 2014 Elsevier B.V. All rights reserved.