A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations

Dauphin LA; Bowen MD

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A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations

机译：从DNA制剂中快速去除炭疽芽孢杆菌孢子的简单方法

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This study establishes a filtration method for the safe removal of Bacillus anthracis spores which may contaminate DNA preparations. Centrifugal filtration with 0.1- mu m filter units can be used following extraction of DNA from B. anthracis spores to render samples safe without compromising the sensitivity of diagnostic real-time PCR assays for B. anthracis.

机译：这项研究建立了一种安全去除可能污染DNA制剂的炭疽芽孢杆菌孢子的过滤方法。从炭疽芽孢杆菌的孢子中提取DNA后，可以使用带有0.1μm过滤器的离心过滤，以确保样品的安全性，而不会影响炭疽芽孢杆菌的实时诊断PCR检测的灵敏度。

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来源
《Journal of Microbiological Methods》 |2009年第2期|共3页
作者
Dauphin LA; Bowen MD;
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原文格式 PDF
正文语种 eng
中图分类微生物学;
关键词
Filters; Filtration; Polymerase chain reaction; Filters; Filtration; Polymerase chain reaction; Spores; Bacillus anthracis;

机译：过滤;过滤;聚合酶链反应;过滤;过滤;聚合酶链反应;孢子;炭疽芽孢杆菌;

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专利

1. A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations [J] . Dauphin LA, Bowen MD Journal of Microbiological Methods . 2009,第2期

机译：从DNA制剂中快速去除炭疽芽孢杆菌孢子的简单方法
2. Novel Sample Preparation Method for Safe and Rapid Detection of Bacillus anthracis Spores in Environmental Powders and Nasal Swabs [J] . Vicki A. Luna, Debra King, Carisa Davis, Journal of Clinical Microbiology . 2003,第3期

机译：安全快速检测环境粉末和鼻拭子中炭疽芽孢杆菌孢子的新样品前处理方法
3. Rapid, high-throughput, culture-based PCR methods to analyze samples for viable spores of Bacillus anthracis and its surrogates [J] . Kane SR, Letant SE, Murphy GA, Journal of Microbiological Methods . 2009,第3期

机译：基于培养的快速，高通量PCR方法，可分析样品中炭疽杆菌及其代用品的活孢子
4. A Simple and rapid method for preparation of Rhizopus Oryzae genomic DNA for PCR analysis [C] . Xinhui Wang, Yin Zhang, Wei Wang Annual International Meeting of the American Society of Agricultural and Biological Engineers . 2014

机译：制备Rhizopus Oryzae基因组DNA的简单且快速的方法，用于PCR分析
5. Evaluation of a novel decontamination and DNA recovery and detection method for Bacillus anthracis. [D] . Bachus, Kristy Ann. 2007

机译：炭疽芽孢杆菌新型去污和DNA回收及检测方法的评估。
6. Novel Sample Preparation Method for Safe and Rapid Detection of Bacillus anthracis Spores in Environmental Powders and Nasal Swabs [O] . Vicki A. Luna, Debra King, Carisa Davis, 2003

机译：安全快速检测环境粉末和鼻拭子中炭疽芽孢杆菌孢子的新样品前处理方法
7. Mechanism of killing of spores of Bacillus anthracis in a high-temperature gas environment, and analysis of DNA damage generated by various decontamination treatments of spores of Bacillus anthracis ,Bacillus subtilis and Bacillus thuringiensis [O] . B. Setlow, S. Parish, P. Zhang, 2014

机译：在高温气体环境中芽孢杆菌血管孢子孢子的机制，以及芽孢杆菌和芽孢杆菌的孢子孢子孢子孢子的各种去污处理产生的DNA损伤分析
8. Surface Layer Protein EA1 is Not a Component of Bacillus anthracis Spores but is a Persistent Contaminant in Spore Preparations [R] . Williams, D. D. , Turnbough, C. L. 2003

机译：表层蛋白Ea1不是炭疽芽孢杆菌孢子的成分，但在孢子制剂中是持久性污染物

A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations

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