EFFECTS OF FLUID SHEAR STRESS AND CIGLITAZONE ON OSTEOBLASTS

摘要

Long-term use of thiazolidinedione (TZD) antidiabetic agents in patients with type 2 diabetes mellitus has been shown to increase the incidence of osteoporosis. Mechanical loading can enhance bone mass by promoting bone formation and suppressing bone resorption, which may be beneficial to patients with TZD-induced osteoporosis. In this study, we examined the cooperative effect of fluid sheax stress (FSS) and ciglitazone (CIG), a type of TZD, on osteo-blasts. The proliferation, osteoblast differentiation-related mRNA expression and translocation of nuclear factor kappaB (NFkappaB) of osteoblasts were assessed. The results show that CIG significantly decreased the proliferation of osteoblasts, inhibited the translocation of NFkappaB to the nucleus and reduced the mRNA expression of COX-2, IGF, Runx2 and OCN. At the same time, CIG also increased the mRNA expression of PPAR7. Conversely, FSS significantly increased the proliferation of osteoblasts, promoted the translocation of NFkappaB to the nucleus and increased the mRNA expression of COX-2, IGF, Runx2 and OCN but decreased the mRNA expression of PPAR7. When FSS and CIG were combined, FSS counteracted the effects of CIG on osteoblasts. Taken together, the current results suggest that FSS is able to arrest the effects of CIG on the proliferation and differentiation of osteoblasts.