Vector competence of Aedes notoscriptus (Diptera: Culicidae) for Barmah Forest virus and of this species and Aedes aegypti (Diptera: Culicidae) for dengue 1-4 viruses in Queensland, Australia

摘要

Aedes notoscriptus colonized from Brisbane, Queensland, Australia, were fed on blood suspensions containing either Barmah Forest (BF) virus, dengue virus type 1 (DEN-1), dengue virus type 2 (DEN-2), dengue virus type 3 (DEN-3), or dengue virus type4 (DEN-4). A. notoscriptus was shown to be moderately susceptible to oral infection with BF virus, ID50=103.3 CCID50 per mosquito, with titres in infected mosquitoes peaking 10 days after infection. BF virus transmission occurred between days 5 and 12, with an average rate of 45% (range, 40-50%). For DEN 1-4, A. notoscriptus showed low level susceptibility to oral infection, with maximum head and thorax squash infection rates of 8, 5, 13 and 3%, respectively. Rates of infection were notably higher in anorth Queensland strain of A. aegypti used as a positive control, with maximum thorax squash infection rates of 58, 68, 78 and 60% for DEN 1-4, respectively. On the basis of these data, it is concluded that A. notoscriptus could act as an efficient vector of BF in urban environments, but it is unlikely to be an important vector of DEN.