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Ovular callus culture studies in Carica papaya L.

机译:番木瓜 L.的卵形愈伤组织培养研究

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摘要

A protocol was developed for initiation and regeneration of ovular callus of pawpaw (cultivars Pusa delicious, Pusa magestic, Sunrise solo, and GK PM-1, and Carica cauliflora). Callus was cultured on full-strength MS medium, half-strength MS medium + 0.5 lmg kinetin/litre, and full-strength MS medium + 0.5 mg kinetin/litre. The ovular callus formation was observed in Pusa delicious x Carica cauliflora at 35 days of age after 2 months of inoculation. Among the culture media, shootlets were observed in half-strength MS medium supplemented with kinetin.
机译:开发了用于启动和再生木瓜卵巢愈伤组织(栽培品种Pusa Delicious,Pusa magestic,Sunrise solo和GK PM-1以及Carica cauliflora )的规程。愈伤组织在全强度MS培养基,半强度MS培养基+ 0.5 mg激动素/升和全强度MS培养基+ 0.5 mg激动素/升上培养。接种2个月后,在35天大的Pusa Delicious x Carica cauliflora中观察到了卵巢的愈伤组织形成。在培养基中,在补充了激动素的半强度MS培养基中观察到了子弹。

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