Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure

摘要

Resveratrol oligomers from the extract of Peaonia lactiflora seeds were investigated for the first time and their potential role as nutraceuticals to treat airway inflammation based on their anti-inflammatory activity examined. After the isolation of five major peaks (suffruticosol B, suffruticosol A, trans-epsilon-viniferin, trans-gnetin H, and cis-suffruticosol D) of a P. lactiflora extract using a one-step high-speed counter current chromatograhy (HSCCC) process, the anti-inflammation activity of each compound was evaluated in the LPS-stimulated RAW264.7 macrophages. Suffruticosol A showed potent levels of inhibition in the expression/production of NO, iNOS, and pro-inflammatory cytokines (TNF-alpha, IL-6, and IL-1 beta) in the assay. Additionally, suffruticosol A exhibited a significant suppression of the increased inflammatory cell count, protein content, pro-inflammatory cytokines and the production of reactive oxygen species in the bronchoalveolar lavage fluid of acute airway inflammation murine model, in which cigarette smoke and LPS exposure were both used as pro-inflammatory stimuli. These experimental results indicate that suffruticosol A has significant effects both in vitro and in vivo to be a potential candidate for airway inflammation therapeutics based on its anti-inflammatory activity. (C) 2015 Elsevier Ltd. All rights reserved.