Genetic Relations and Identification of Genus Juglans Using I-SSR Markers

摘要

Using 104 fragments amplified from 9 I-SSR primers, a total of 83 cultivars in Juglans spp. including new cultivars used for the present study were identified. The new cultivars 'Suwon No.l', 'Suwon No.2', and 'Wanghodo' are hybrids between the domestic cultivars and the introduced cultivars such as 'Concord' and 'McKinster' bearing big nuts. Consequently, these cultivars were closer to the hybrid between 'Sansung 4xConcord' than to domestically selected cultivars such as 'Sangchon 4' from the viewpoint of genetic relationship. The domestic cultivars such as 'Sangchon 2', 'Sangchon 4', and 'Sansung 4' belonged to the same group and were genetically distinctive from the introduced cultivars. This suggests that there would be difference in genetic structure between the domestic and the introduced cultivars. The cultivar 'Yeongdong' was clustered into the group of hybrids between the domestic and the introduced cultivars although it was developed by the selection of a tree from a domestic population.This might be because the genes were introduced from the introduced cultivars into the domestic population via open-pollination. The 'Concord x Sangchon 1', 'Concord x Sangchon 2', and ' Concord x Daebu 1' were clustered into the same group whereas 'Lakex Sangchon 2' was clustered into the different group. This indicates that the genetic characteristics of hybrids may be influence more strongly by the mother tree than by the father tree.