Somatic Embryo Germination and the Related Biochemical Changes of Liriodendron tulipifera by Bioreactor Immersion Time

摘要

To determine physical and physiological factors for Liriodendron tulipifera L. somatic embryo germination, temporary immersion bioreactor (TIB) system was investigated. It was designed to immerse liquid media with plantlets so that it was able to adjust the immersion time. Immersion of 120 minutes every 4 hours and 60 minutes every 4 hours was found to be effective in germination (91.64%, 85.67%, respectively). However, hyperhydricity of the plantlets was higher in short immersion time (15 minutes every 6 hours) and long immersion time (120 minutes every 4 hours) (51.61%, 34.28%, respectively). Immersion of 60 minutes every 4 hours showed the lowest hyperhydric plantlets, and also it showed the lowest activities of abscisic acid (ABA), superoxide dismutase (SOD), and catalase. The overall results implied that immersion time of media affected germination and growth of somatic embryo, and it was able to make use of germination and growth of L. tulipifera somatic embryos.