Synthesis of selectively labeled fatty acids and tritium labeled acyl-CoA and CoN

摘要

CoA and acyl-CoAs of high specific activity were needed in studies of a low-activity enzyme system in brain.1 The current work is devoted to tritium labeling of different acyl-CoA at acyl or CoA fragment.In order to introduce tritium into the unsaturated long-chain acyl part, the desired [3H]fatty acid is usually prepared by selective hydrogenation of its acetylenic precursor. However, in some cases simpler ways can be used namely, the selective hydrogenation of the often easily available polyunsaturated fatty acids.