Evaluation of ~( 99m)Tc-immunoglobulins for imaging infection in the rat

摘要

Three immunoglobulin molecules were evaluated as infection imaging agents in a rat model of S. aureas infection: ~( 99m)Tc-infliximab, ~( 99m)Tc-human immunoglobulin (HIG) and ~( 99m)Tc-rat immunoglobulin (RIG). Infliximab is a chimeric monoclonal antibody specific for human tumour necrosis factor alpha (TNFa). ~( 99m)Tc-HIG was chosen as an exogenous protein and ~( 99m)Tc-RIG as an endogenous marker Each immunoglobulin was treated with 2-mercaptoethanol and the reduced antibody was isolated by size exclusion chromatography. In combination with Sn~(II)-methylenediphosphonic acid, cold kit formulations were prepared. Native and reduced infliximab were tested for rat TNFa binding ability in vitro.