Synthesis of deuterium-labelled coenzyme Q10

摘要

Deuterium-labelled coenzyme Q10 ([2-CD3-1'-CD2]coenzyme Q10, coenzyme Q10-d5) was synthesized by condensation of 2,3-dimethoxy-[5-CD3]methyl-1, 4-hydroquinone with [1-CD2]decaprenol. Five positions were selected for deuteration as replacement at these positions allowed examination of every step of the synthesis. This examination was carried out by a combination of 1H- and 13C-nuclear magnetic spectrometry and mass spectrometry. Further, these positions have been proved to be metabolically stable. This reagent makes simultaneous quantification of the source of coenzyme Q10 (exogenously supplied or endogenously supplied) possible in biological samples by measurements on gas chromatography-mass spectrometry. Copyright < copyright > 2002 John Wiley & Sons, Ltd.