Simple single-step single-enzyme synthesis of [ 14C]-GSH

摘要

The tri-peptide [ 14C]-glutathione ([ 14C]-GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L-γ-glutamyl-L-cysteine and [ 14C]-glycine. Preparative reverse phase HPLC afforded [ 14C]-GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [ 14C]-GSH to the disulfide [ 14C]-GSSG for storage are discussed.