Enzymatic synthesis of isotopomers of tyramine labeled with deuterium and tritium

摘要

The combined chemical and enzymatic methods of synthesis of five isotopomers of L-tyrosine, L-Tyr, and their derivatives, i.e. corresponding isotopomers of tyramine (TA), labeled with deuterium and tritium have been reported. Two-step synthesis consists with introduction of deuterium or tritium label into intermediate L-Tyr using isotope exchange followed by enzymatic decarboxylation using enzyme tyrosine decarboxylase (EC 4.1.1.25). This way five isotopomers of L-tyrosine, i.e. [2-~2H]-L-, [2-~3H]-L-,[2-~2H/~3H]-L-, [3',5'-~2H_2]-L-,[3',5'-~3H_2]-L-Tyr, and six isotopomers of tyramine i.e. [1S-~2H]-, [1S-~3H]-, [1S-~2H/~3H]-, [3',5'-~2H_2]-, [3',5'-~2H_2]-, [2',6'-~2H_2]-TA were obtained.