DIABETIC INTERSTITIAL GLUCOSE IN THE SKINTISSUE BY ATR-FTIR SPECTROSCOPY VERSUSCAPILLARY BLOOD GLUCOSE

摘要

Recently introduced horizontal attenuated total reflectance (HATR) Fourier transform infrared(FTIR) spectroscopy for real-time assessment and continuous monitoring of glucose biomoleculesin the skin tissue directly on the patients might appear a promising alternative to interpretthe activity of interstitial glucose metabolism in vivo by means of evaluating the dynamics ofchanges of glucose concentrations in interstitial fluid (IF). In the present study, in vivo spectraby ATR-FTIR spectroscopy were obtained post-prandially during a 120-180-minute continuousmonitoring in three patients with type 2 diabetes and compared to pre-prandial spectra. Inall patients with diabetes interstitial glucose levels at 1030 and 1041 cm-1 reflected the bestrelationship with blood glucose. The lag time (LT) required for glucose to diffuse from thecapillary to epidermal skin tissue was calculated between 0 and 60 minutes at all measured glu-cose biomolecules. Data showed intra- and inter-subject variations of each glucose biomolecule,pointing to similarities and differences among interstitial glucose metabolism of the patients.Finally, the findings suggest that HATR-FTIR spectroscopy might have the potential for clini-cal interpretation of activity of glucose metabolism for diagnosis, management, and treatmentof patients with diabetes.