Synthesis, interaction with double-helical DNA and biological activity of the water soluble complex cis-dichloro-1,2-propylenediamine-N,N,N ',N '-tetraacetato ruthenium (III) (RAP)

摘要

The effects exerted by the new complex cis-dichloro-1,2-propylenediaminetetraacetato ruthenium (111), H[RuCl2(PDTA-H-2)] [1, RAP], on DNA and cultured tumor cells (ovarian carcinoma TG cell line) were studied. The comparative study of circular dichroism (CD) spectra obtained from DNA and RAP DNA system evidences the interaction of the complex with DNA. Compound I also interacted with tumor TG cells to slow their proliferation rate. BrdU incorporation was enhanced in cells treated with compound 1, as evidenced by a single-cell electrophoresis method (comet assay), in accordance with RAP-induced DNA damage. DNA migration of compound 1-treated cells was similar to that induced by noxious agents other than cross-linking chemicals. The stability of [RUCl2(PDTA-H-2)]-DNA binding is suggested by the high degree of damage that persisted after removal of compound I from the culture medium. (c) 2006 Elsevier Inc. All rights reserved.