Formation of CoQ10 reduced form by mixing CoQ10 oxidized form γCD complex and vitamin C in powder

摘要

We have already reported the enhancement of the stability and bioavailability of coenzyme Q10 (CoQ10) oxidized form by γ-cyclodextrin (γCD) complexation. In a series of the studies, we investigated an easy and economical conversion of CoQ10 oxidized form to its reduced form in complex powder, using inexpensive vitamin C (VC) as the reductant. CoQ10 oxidized form or its γCD complex and VC were physically mixed at the molar ratio of 1:0 to 1:50. The mixtures were stored at 60 °C and 75% RH. The sampling was made at certain interval, and both CoQ10 oxidized and reduced form contents were measured by high performance liquid chromatography (HPLC). The result shows that the conversion ratio to CoQ10 reduced form in γCD complex was significantly higher than that of non-inclusion compound (ca. 80% versus ca. 30% at the maximum). It was also confirmed that CoQ10 reduced form in γCD complex remains as stable as its oxidized form in γCD complex. Free radical scavenging potential of partially reduced CoQ10-γCD complex was assayed with 1,1-diphenyl-2-picrylhydrazyl (DPPH).