DNA cleavage promoted by Cu~(2+)complex of cyclen containing pyridine subunit

摘要

The tetraazamacrocycle crown ether(cyclen)containing two pyridine subunits was prepared by a modified procedure and the interaction of its metal complexes with DNA was studied by agarose gel electrophoresis analysis.The results indicate that the Cu~(2+)complex as nuclease model can promote the hydrolysis of phosphodiester bond of supercoiled DNA.The rate of degradation of the supercoiled DNA(form I)to nicked DNA(form II)obtained at physiological condition in the presence of 2.14 mM Cu~(2+)complex is 2.31 x 10~(-3)min~(-1).The dependence of the rate of supercoiled DNA cleavage from the complex concentration shows an unusual profile and a hydro-lytic cleaving mechanism of two monometallic complexes through cooperation from two-point binding to DNA is proposed.