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首页> 外文期刊>Journal of Horticultural Research >ASSESSMENT OF GENETIC DIVERSITY IN CULTIVATED TOMATO(SolanumlycopersicumL.) GENOTYPES USING RAPD PRIMERS
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ASSESSMENT OF GENETIC DIVERSITY IN CULTIVATED TOMATO(SolanumlycopersicumL.) GENOTYPES USING RAPD PRIMERS

机译:利用RAPD评估野生番茄(Solanumlycopersicuml。)基因型的遗传多样性

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Random Amplified Polymorphic DNA (RAPD) analysis was carried out on 19 Azerbaijan tomato genotypes, both cultivars and local populations. A total of 26 amplified products were revealed by 6 primers. The genetic similarity among evaluated genotypes ranged from 0.188 to 1.000. The lowest similarity was observed between cultivars 'Azerbaijan' and 'Shakar' (0.188), while the highest between 'El-nur' and 'Garatag' (1.000). The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on Jaccard's similarity coefficient divided genotypes into four main groups. The first group was the largest and consisted of 12 genotypes, while the fourth group was the smallest consisted of 1 genotype only. The most polymorphic primer was OPB-18 that presented a genetic diversity index of 0.823, while the least informative was primer OPG-17 with an index of 0.349. The average genetic diversity calculated from RAPD data was 0.665.
机译:对19个阿塞拜疆番茄基因型(品种和当地种群)进行了随机扩增多态性DNA(RAPD)分析。用6个引物揭示了总共26个扩增产物。被评估基因型之间的遗传相似性在0.188至1.000之间。品种'阿塞拜疆'和'Shakar'之间的相似性最低(0.188),而'El-nur'和'Garatag'之间的相似性最高(1.000)。基于Jaccard相似系数的不加权算术平均成对分组(UPGMA)聚类分析将基因型分为四个主要组。第一组最大,由12个基因型组成,而第四组最小,仅由1个基因型组成。多态性最高的引物是OPB-18,其遗传多样性指数为0.823,而信息最少的是引物OPG-17,其指数为0.349。根据RAPD数据计算得出的平均遗传多样性为0.665。

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