首页> 外文期刊>Journal of Hepatology: The Journal of the European Association for the Study of the Liver >Impairment of natural killer cell and dendritic cell functions by the soluble form of MHC class I-related chain A in advanced human hepatocellular carcinomas.
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Impairment of natural killer cell and dendritic cell functions by the soluble form of MHC class I-related chain A in advanced human hepatocellular carcinomas.

机译:MHC I类相关链A的可溶性形式在晚期人类肝细胞癌中损害自然杀伤细胞和树突状细胞的功能。

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摘要

BACKGROUND/AIMS: MHC class I-related chain A (MICA), a human ligand of natural killer (NK) cell stimulatory receptor NKG2D, is expressed in human hepatocellular carcinomas (HCC). Earlier research demonstrated that the soluble form of MICA (sMICA) is released from some types of tumors, but its presence and role in HCC was not determined. METHODS: Serum sMICA was studied in 26 patients with HCC. In vitro experiments were performed to examine the impact of sMICA on NK cell expression of NKG2D and subsequent dendritic cell (DC) activation. RESULTS: The levels of sMICA were frequently elevated in patients with advanced HCC. The elevation of sMICA was associated with down-regulated NKG2D expression and impaired activation of NK cells. In vitro experiments revealed that sMICA derived from advanced HCC was responsible for down-modulation of NKG2D expression and NK cell functions. NK cells upon stimulation of human hepatoma cells induced maturation of DC and enhanced the allostimulatory capacity of DC; maturation and activation of DC were completely abolished when NK cells were pre-treated with sMICA-containing serum. CONCLUSIONS: sMICA is present in sera of patients with advanced HCC and may serve as a tumor evasion mechanism by negatively modulating both innate and adaptive immunity.
机译:背景/目的:MHC I类相关链A(MICA)是自然杀伤(NK)细胞刺激受体NKG2D的人类配体,在人类肝细胞癌(HCC)中表达。较早的研究表明,MICA(sMICA)的可溶形式是从某些类型的肿瘤中释放出来的,但尚未确定其在肝癌中的存在和作用。方法:对26例HCC患者的血清sMICA进行了研究。进行了体外实验以检查sMICA对NKG2D NK细胞表达和随后的树突状细胞(DC)激活的影响。结果:晚期肝癌患者的sMICA水平经常升高。 sMICA的升高与NKG2D表达下调和NK细胞活化受损有关。体外实验表明,源自高级肝癌的sMICA负责NKG2D表达和NK细胞功能的下调。 NK细胞在刺激人肝癌细胞后诱导DC的成熟并增强DC的同素刺激能力。用含sMICA的血清对NK细胞进行预处理后,DC的成熟和DC活化完全消失了。结论:sMICA存在于晚期HCC患者的血清中,并且可能通过负调节先天免疫和适应性免疫而充当肿瘤逃逸机制。

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