Amplification of background EMG activity affects the interpretation of H-reflex gain

摘要

In many H-reflex studies, the modulation of the H-reflex is usually compared relative to the normal EMG activity within the muscle. Such comparisons enable the investigators to infer whether the change in the amplitude of the H-reflex was independent of normally occurring muscle activity. This interpretation of the H-reflex is regarded as H-reflex gain, a popular dependent variable in human H-reflex studies. However, in many studies to date, the muscle activity level has been determined from the same EMG signal from which the H-reflex is recorded. This leads to an important methodological consideration: measuring the ongoing normal EMG activity from the same signal might result in an inaccurate measurement, since this EMG signal will need to be minimally amplified to capture the synchronous volley of the H-reflex amplitude. In this study we examined this possibility and found that comparing the EMG activity level from the seated position to standing position yields different results (on average 8.03% in the measurement of the increase of muscle activity). This difference was both dependent on the task and also on the EMG instrumentation used. To solve this problem we suggest the bifurcation of the EMG signal from the recording electrodes with differential amplification of the signal. With this method, both the naturally occurring muscle activity and the H-reflex signal are collected from the same area of the muscle and a more accurate measurement of the H-reflex gain will be yielded.