A Simple Screening Method for Anti-attachment Compounds Using Monospores of Porphyra yezoensis Ueda

摘要

We measured the anti-attachment activity of allelochemical and antifouling substances using monospores from Porphyra yezoensis Ueda as an assay. Methanol or aqueous extracts (20 μg/mL) from 32 seaweeds were added to monospore suspensions. Methanol extracts of Corallina pilulifera, Ishige sinicola, Sargassum horneri, and Sargassum sagamianum inhibited attachment by >90% compared to the reference. Phenolic compounds fractionated from 5. sagamianum caused the most potent inhibition. P. yezoensis monospores also showed significant sensitivity to known allelochemical and algicidal compounds.