Y Chromosome STR Haplotypes and Allele Frequencies in a Southern Indian Male Population

摘要

Bloodstain samples were obtained from 123 Southern Indian males. DNA was extracted from each sample using a standard SDS/proteinase K extraction buffer. Each sample was isolated using Microcon~(TM) 100 filters (Millipore Corporation, Bedford, MA) following phenol-chloroform-isoamyl alcohol treatment of the DNA extract and quantified . using the Quantifiler~(TM)Human real time PCR assay (Applied Biosystems, Foster City, CA). Approximately 0.75 ng of DNA was targeted for amplification of the Y-short tandem repeats (STR) loci DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS438, DYS439, and DYS437 using the PowerPlex~R Y PCR Amplification Kit (1) according to manufacturers' recommendations (2) (Promega Corporation, Madison, WI). PCR products generated were analyzed on the ABI PRISM~(TM) 310 Genetic Analyzer (Applied Biosystems). Haplotypes were defined using Genescan~(TM) and Genotyper~(TM) software (Applied Biosystems) and the PowerTyperY macro from PowerTyper Release 2 (Promega Corporation).