Population data on HLA-DQA1, LDLR, GYPA, HBGG, D7S8, and GC PCR-based loci in Serbia.

摘要

A series of 131 samples was collected from 131 unrelated individuals living in Serbia (100 whole-blood samples and 31 teeth extracted for therapeutic purposes). The DNA was isolated using proteinase K digestion, followed by phenol-chloroform-isoamyl extraction and ethanol precipitation. Purification of DNA was performed using centricon 100 (Millipore Corporation, Bedford, MA), DNA amplification was performed using AmpliType HLA-DQA1+PM, and typing of alleles was performed by reverse dot hybridization with the Typing Kit, all according to the manufacturer's protocol (Applied Biosystems, Foster City, CA).