Genetic polymorphism of two novel STR loci AY639920 and AY639923 in a Chinese population.

摘要

Blood samples were collected from unrelated individuals of the Chinese Han ethnic group in Chengdu of China. DNA was extracted using the Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/AY639920.htm and http://www.fayi.cn/dna/AY639920.htm. The volume of PCR reaction for each locus was 37.5 uL. The PCR products were analyzed by vertical nondenaturing polyacrylamide gel electrophore-sis with a continuous buffer system and visualized by silver staining (2,3). Data were analyzed using POWERSTATS program (4). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (5) and no deviation from Hardy-Weinberg equilibrium was observed (Tables 1 and 2).