Polymorphism of three short tandem repeat loci in Chinese population.

摘要

Whole blood samples were obtained from unrelated individuals of Chinese Han ethnic group in Chengdu of China. Genomic DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.31studio.com/dna. The volume of PCR reaction for each locus was 37.5 muL. The amplified products were separated by horizontal nondenaturing polyacryl-amide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed using POWERSTATS program (3). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (4). No deviation from Hardy-Weinberg equilibrium was observed.