Development of an enzyme-linked immunosorbent assay for detection of ofloxacinresidue in environment matrix

摘要

Ofloxacin polyclonal antibody was prepared and cross-reactivity to other fluroquinones was observed. Further, a rapid, low-cost ELISA analytical technique was developed, with IC50 at 1.2 ng.mL', for the analysis of ofloxacin in the environment matrixincluding wastewater, manure and sludge. The mean recoveries and coefficients of variation were in a range of 74-83% and 8.5-12.2%, respectively, in wastewater. The mean recoveries and coefficients of variation in pig manure and sludge were from 62% to 78% and from 8.4% to 14.4%, respectively. Concentrations of ofloxacin using ELISA were sometimes greater than measured with LC-MS/MS. It may be due to cross-reactivity to structurally similar compounds or metabolites. Results from this study showed that the ELISA can be used as a preliminary screening tool for later instrumental analysis.